解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::A glycosyltransferase was fused to the yeast cell wall protein Pir, which forms the Pir1-4 protein family and is incorporated into the cell wall by an unknown linkage to be displayed at the yeast cell surface. We first expressed the PIR1-HA-gma12+ fusion, in which gma12+ encodes alpha-1,2-galactosyltransferase from th...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwg014
更新日期:2003-02-01 00:00:00
abstract::Binding characteristics of two types of ligands for human neo-C-reactive protein (neo-CRP), which is a conformationally altered but physiologically relevant form of CRP, were studied fluorometrically by probing CRP immobilized on a polystyrene surface with europium-labeled ligands. Two Eu-ligands used were bovine seru...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwg002
更新日期:2003-01-01 00:00:00
abstract::The synthesis of Galalpha1-3Gal-terminated oligosaccharides (alpha-Gal) epitopes has been interrupted during the course of evolution, starting with Old World primates. Partial sequences similar to the alpha1,3-galactosyltransferase (alpha1,3GalT) gene, which governs the synthesis of alpha-Gal epitopes, have been detec...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwf087
更新日期:2002-12-01 00:00:00
abstract::Heparan sulfates (HSs) exert critical regulatory actions on many proteins, including growth factors, and are essential for normal development. Variations in their specific sulfation patterns are known to regulate binding and signaling of fibroblast growth factors (FGFs) via tyrosine kinase receptors (FGFRs). We previo...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwf072
更新日期:2002-11-01 00:00:00
abstract::We report the identification, molecular cloning, and characterization of an endo-beta-N-acetylglucosaminidase from the nematode Caenorhabditis elegans. A search of the C. elegans genome database revealed the existence of a gene exhibiting 34% identity to Mucor hiemalis (a fungus) endo-beta-N-acetylglucosaminidase (End...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwf073
更新日期:2002-10-01 00:00:00
abstract::Assembly of a protective cyst wall by Giardia is essential for the survival of the parasite outside the host intestine and for transmission among susceptible hosts. The structure of the G. intestinalis filamentous cyst wall was studied by chemical methods, mass spectrometry, and (1)H nuclear magnetic resonance spectro...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwf059
更新日期:2002-08-01 00:00:00
abstract::N-acetylglucosaminyltransferase V, encoded by the Mgat5 gene, plays an important regulatory role in the synthesis of complex N-glycans, including the Lewis antigens. The elevated expression of this enzyme is regulated during development and is highly associated with cellular transformation and malignancy. In addition,...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwf040
更新日期:2002-07-01 00:00:00
abstract::The content of sulfated glycans having 6-O-sulfated GlcNAc residues alters in the course of colonic carcinogenesis. We previously characterized two GlcNAc 6-O-sulfotransferases (SulTs), SulT-a and -b, expressed in colonic normal tissues and adenocarcinomas [Seko et al. (2000) Glycobiology, 10, 919-929]. Levels of the ...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/12.6.379
更新日期:2002-06-01 00:00:00
abstract::Algal fucoidan is an alpha-L-fucose-based polysaccharide endowed with important biological properties for which the structure has not yet been fully elucidated. In an attempt to implement new enzymatic tools for structural study of this polysaccharide, we have found a fucosidase activity in the digestive glands of the...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/12.4.273
更新日期:2002-04-01 00:00:00
abstract::We have characterized galectin family proteins in adult tissues of Xenopus laevis and purified 14-kDa and 36-kDa proteins from the liver. The liver galectins showed comparable hemagglutination activities to those of mammalian galectins. Furthermore, we isolated five galectin cDNAs from a Xenopus liver library. These c...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/12.3.163
更新日期:2002-03-01 00:00:00
abstract::In this study, we report the first cloning and characterization of a N-acetylneuraminic acid phosphate synthase gene from Drosophila melanogaster, an insect in the protostome lineage. The gene is ubiquitously expressed at all stages of Drosophila development and in Schneider cells. Similar to the human homologue, the ...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/12.2.73
更新日期:2002-02-01 00:00:00
abstract::The high-molecular-mass salivary mucin MG1, one of two major mucins produced by human salivary glands, plays an important role in oral health by coating the tooth surface and by acting as a bacterial receptor. Here this mucin was purified from the submandibular/sublingual saliva of a blood group O individual. The pres...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/12.1.1
更新日期:2002-01-01 00:00:00
abstract::A genetic basis for the biosynthetic assembly of the globotetraose containing lipopolysaccharide (LPS) of Haemophilus influenzae strain RM118 (Rd) was determined by structural analysis of LPS derived from mutant strains. We have previously shown that the parent strain RM118 elaborates a population of LPS molecules mad...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/11.11.957
更新日期:2001-11-01 00:00:00
abstract::An ultrasensitive method for analysis of polysialic acid (polySia) chains, using fluorescence-assisted high-performance liquid chromatography was developed. The new method is a substantial improvement of our earlier method in which the reducing terminal Sia residues of a homologous series of oligo/polySia hydrolytical...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/11.9.759
更新日期:2001-09-01 00:00:00
abstract::Beta-1,2-linked mannosides from Candida albicans phosphopeptidomannan (PPM) bind to macrophages through a receptor independent from the macrophage alpha-linked mannose receptor and stimulate these cells to secrete immune mediators. Anti-beta-1,2-linked mannoside but not anti-alpha-linked mannoside antibodies produced ...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/11.8.693
更新日期:2001-08-01 00:00:00
abstract::Gangliosides are able to bind to the epidermal growth factor receptor and inhibit its activation, but the mechanism of this inhibition is unknown. To address the role of receptor carbohydrates in facilitating interaction with gangliosides, we examined the ability of GM3 to bind the deglycosylated receptor and inhibit ...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/11.7.515
更新日期:2001-07-01 00:00:00
abstract::We show by nanoelectrospray ionization (nanoES) Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR MS) that it is possible to observe oligosaccharide-protein complexes with dissociation constants in the millimolar range, such as P(k) trisaccharide (globotriaoside) complexed with the Shiga-like toxin (...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/11.7.605
更新日期:2001-07-01 00:00:00
abstract::Ceramide is a pivotal molecule in signal transduction and an essential structural component of the epidermal permeability barrier. The epidermis is marked by a high concentration of ceramide and by a unique spectrum of ceramide species: Besides the two ceramide structures commonly found in mammalian tissue, N-acylsphi...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/11.6.451
更新日期:2001-06-01 00:00:00
abstract::The glycobiology of Schistosoma mansoni is dominated by developmentally regulated expression of various fucosylated structures, most notably the Lewis X epitope and a multifucosylated sequence, Fuc alpha1-->2Fuc alpha1-->, in its various forms. For the infective cercarial stage, Lewis X has been structurally identifie...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/11.5.395
更新日期:2001-05-01 00:00:00
abstract::Two kinds of molecules, one recognizing the sialo-/asialo-N-acetyllactosamine structures and the other recognizing the Lewis X structure in a divalent cation-independent manner, were detected on the head of boar sperm prepared from cauda epididymis by fluorescence-labeled or biotinylated dextran-based multivalent olig...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/11.4.313
更新日期:2001-04-01 00:00:00
abstract::We tested the importance of the aspartate-any residue-aspartate (DXD) motif for the enzymatic activity and nucleotide binding capacity of the Golgi glycosyltransferase GM2 synthase. We prepared point mutations of the motif, which is found in the sequence 352-VLWVDDDFV, and analyzed cells that stably expressed the muta...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/11.3.217
更新日期:2001-03-01 00:00:00
abstract::Streptococcus macedonicus is a Gram positive lactic acid bacterium that is part of the starter flora present in Greek sheep and goat cheeses. The S. macedonicus Sc136 strain produces a high-molecular-mass, highly texturizing exopolysaccharide composed of D-glucose, D-galactose, and N-acetyl-D-glucosamine in the molar ...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/11.2.131
更新日期:2001-02-01 00:00:00
abstract::Recent studies have shown that O-mannosyl glycans are present in several mammalian glycoproteins. Although knowledge on the functional roles of these glycans is accumulating, their biosynthetic pathways are poorly understood. Here we report the identification and initial characterization of a novel enzyme capable of f...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/11.1.37
更新日期:2001-01-01 00:00:00
abstract::alpha-1,4-Glucan lyase cleaves alpha-1,4-linkages of nonreducing termini of alpha-1,4-glucans to produce 1,5-anhydrofructose (1,5-AnFru). The enzymes isolated from fungi and algae show high homology with glycoside hydrolase family 31. Purification of alpha-1,4-glucan lyase from rat liver using DEAE Cellulose chromatog...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/10.12.1283
更新日期:2000-12-01 00:00:00
abstract::The interaction of heparan sulfate (HS) (and the closely related molecule heparin) with FGF-1 is a requirement for enabling the growth factor to activate its cell surface tyrosine kinase receptor. However, little is known about the regulatory role of naturally occurring cell surface HS in FGF-1 activation. We have add...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/10.11.1183
更新日期:2000-11-01 00:00:00
abstract::Fucosylation of glycans on glycoproteins and -lipids requires the enzymatic activity of relevant fucosyltransferases and GDP-L-fucose as the donor. Due to the biological importance of fucosylated glycans, a readily accessible source of GDP-L-fucose would be required. Here we describe the construction of a stable recom...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/10.10.1041
更新日期:2000-10-01 00:00:00
abstract::Type A Pasteurella multocida, an animal pathogen, employs a hyaluronan [HA] capsule to avoid host defenses. PmHAS, the 972-residue membrane-associated hyaluronan synthase, catalyzes the transfer of both GlcNAc and GlcUA to form the HA polymer. To define the catalytic and membrane-associated domains, pmHAS mutants were...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/10.9.883
更新日期:2000-09-01 00:00:00
abstract::The N-glycans of human serum transferrin produced in Trichopulsia ni cells were analyzed to examine N-linked oligosaccharide processing in insect cells. Metabolic radiolabeling of the intra- and extracellular protein fractions revealed the presence of multiple transferrin glycoforms with molecular weights lower than t...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/10.8.837
更新日期:2000-08-01 00:00:00
abstract::Sialic acid has long been considered to be the sole receptor for influenza virus. The viral hemagglutinin (HA) is known to bind cell surface sialic acid, and sialic acids on viral glyco-proteins are cleaved by the viral neuraminidase (NA) to promote efficient release of progeny virus particles. However, NWS-Mvi, a mut...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/10.7.649
更新日期:2000-07-01 00:00:00
abstract::Hyaluronan (HA) is a linear charged polysaccharide whose structure is made up of repeating disaccharide units. Apparently conflicting reports have been published about the nature of the helical structure of HA in the solid state. Recent developments in the field of molecular modeling of polysaccharides offer new oppor...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/10.6.587
更新日期:2000-06-01 00:00:00
abstract::Erythropoietin (Epo) is a 166 amino acids protein containing three N-glycosylation sites (Asn-24, Asn-38, and Asn-83) and 1 O- glycosylation site (Ser-126) and involved in the regulation of the level of red blood cells. Today, only one recombinant human Epo (rHuEpo), produced in CHO cell line, is extensively used in t...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/10.5.511
更新日期:2000-05-01 00:00:00
abstract::Natural human interferon (IFN)-gamma has mainly biantennary complex-type sugar chains and scarcely has multiantennary structures. We attempted to remodel the sugar chain structures using IFN-gamma as a model glycoprotein. To obtain the branching glycoforms of IFN-gamma, we introduced the genes for GnT-IV (UDP-N-acetyl...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/10.4.421
更新日期:2000-04-01 00:00:00
abstract::The substrate specificity of an alpha2,3-sialyltransferase (v-ST3Gal I) obtained from myxoma virus infected RK13 cells has been determined. Like mammalian sialyltransferase enzymes, the viral enzyme contains the characteristic L- and S-sialyl motif sequences in its catalytic domain. Analysis of the deduced amino acid ...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/10.3.313
更新日期:2000-03-01 00:00:00
abstract::Diabetes mellitus was induced in one group of rats by a single injection of streptozotocin. The glycemia, the body weight, and the blood systolic pressure were measured every week, and the 24 h urine volume and urinary excretions of creatinine, albumin and glycosaminoglycans were measured every 2 weeks. At the end of ...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/10.2.185
更新日期:2000-02-01 00:00:00
abstract::We show here that cell surface glycosaminoglycans (GAGs) are involved in the binding of stromal cell-derived factor (SDF)-1alpha to CD4(+)lymphoid CEM or monocytic U937 cells, inasmuch as pretreating the cells with heparitinase or chondroitinase inhibits SDF-1alpha binding by 40-41% and 31-35%, respectively. Soluble h...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/10.1.21
更新日期:2000-01-01 00:00:00
abstract::The cDNAs that encode the 70 kDa C-terminal portion of Plasmodium falciparum merozoite surface protein 1 (MSP-1), with or without an N-terminal signal peptide sequence and C-terminal glycosylphosphatidylinositol (GPI) signal sequence of MSP-1, were expressed in mammalian cell lines via recombinant vaccinia virus. The ...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/9.12.1347
更新日期:1999-12-01 00:00:00
abstract::We have examined the time course of the neuronal death and regeneration of rat axotomized hypoglossal nerve with various conditions of the nerve resection, and established a useful system to measure neurotrophic activities of bioactive substances. In this system, neuronal death can be evaluated by counting surviving n...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/9.11.1247
更新日期:1999-11-01 00:00:00
abstract::Aspartic protease, widely used as a milk-coagulating agent in industrial cheese production, contains three potential N-glycosylation sites. In this study, we report the characterization of N-linked oligosaccharides on recombinant aspartic protease secreted from the methylotrophic yeast Pichia pastoris using a combinat...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/9.10.1037
更新日期:1999-10-01 00:00:00
abstract::Two different types of peptide:N-glycanase (PNGase) were identified in developing embryos of medaka fish ( Oryzias latipes ). Because the optimum pH values for their activities were acidic and neutral, they were designated as acid PNGase M and neutral PNGase M, respectively. The acid PNGase M corresponded to the enzym...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/9.9.887
更新日期:1999-09-01 00:00:00
abstract::The oligosaccharide chains (glycans) attached to cell surface and extracellular proteins and lipids are known to mediate many important biological roles. However, for many glycans, there are still no evident functions that are of obvious benefit to the organism that synthesizes them. There is also no clear explanation...
journal_title:Glycobiology
pub_type: 杂志文章,评审
doi:10.1093/glycob/9.8.747
更新日期:1999-08-01 00:00:00